Coagglutination for rapid testing of toxin producing Corynebacterium diphtheriae.
Artigo
em Inglês
| IMSEAR
| ID: sea-17475
ABSTRACT
Coagglutination test was standardised for rapid detection of diphtheria toxin after primary culture of C. diphtheriae isolates on conventional Loeffler's medium. The procedure was optimised with diphtheria toxoid (DT)-antidiphtheritic serum (ADS) system wherein Cowan-I strain of Staphylococcus aureus was sensitised with ADS. Toxoid in the range of 1.02 lf/ml to 4200 lf/ml could be detected by the present procedure. Toxin of C. diphtheriae could be demonstrated in five of eight clinical isolates, this correlated with the conventional guinea pig pathogenicity test. The coagglutination test thus needs further confirmation of its potential as a simple and satisfactory test for rapid detection of toxin-producing C. diphtheriae.
Texto completo:
DisponíveL
Índice:
IMSEAR (Sudeste Asiático)
Assunto principal:
Fatores de Tempo
/
Humanos
/
Testes de Aglutinação
/
Corynebacterium diphtheriae
/
Meios de Cultura
/
Difteria
/
Toxina Diftérica
/
Cobaias
/
Animais
Idioma:
Inglês
Ano de publicação:
1993
Tipo de documento:
Artigo
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