Optimization of Production of Fructooligosaccride using Aureobasidium pullullans.

ABSTRACT

This present study focuses on the microbial production of Fructosyl Transferase (FTase) and the production of Fructooligosaccharides(FOS) by transfructosylation using this enzyme. Fructooligosaccharides are industrially produced from sucrose by microbial enzymes with transfructosylating activity. Fructosyltransferase is produced intracellulraly by fungi. In the present study fungus Aureobasidium pullulans was used to produce FOS and effect of different physical and chemical parameters on FOS production were studied. The analysis of the product was performed by High Pressure Liquid Chromatography (HPLC).