Identification & differentiation of Mycobacterium avium & M. intracellulare by PCR- RFLP assay using the groES gene.
Artigo
em Inglês
| IMSEAR
| ID: sea-17776
ABSTRACT
BACKGROUND & OBJECTIVE:
We report a new polymerase chain reaction (PCR) - restriction fragment length polymorphism (RFLP) assay using mycobacterial groES as a target to identify Mycobacterium avium and M. intracellulare in clinical samples.METHODS:
The assay was standardized using M. avium and M. intracellulare standard strains obtained from ATCC and was tested with 45 M. avium-M. intracellulare complex (MAC) clinical isolates (Of which 31 were from HIV(+) individuals). The standard and clinical strains were typed with HPLC based mycolic acid fingerprinting.RESULTS:
Three polymorphisms (BamHI, BstNI and HgaI) were identified for inter-species differentiation among standard strains; of which, only HgaI was found to be useful in clinical isolates. Of the 45 isolates, 25 were M. avium and 20 were M. intracelluare. MAC isolates, which could not be differentiated by HPLC analysis, were also typed by this method. INTERPRETATION &CONCLUSION:
The use of mycobacterial groES as a PCR-RFLP target for M. avium and M. intracellulare is a simple and rapid method that can complement HPLC in their differentiation.
Texto completo:
DisponíveL
Índice:
IMSEAR (Sudeste Asiático)
Assunto principal:
Especificidade da Espécie
/
Proteínas de Bactérias
/
Polimorfismo de Fragmento de Restrição
/
Humanos
/
DNA Bacteriano
/
Sequência de Bases
/
Complexo Mycobacterium avium
/
Reação em Cadeia da Polimerase
/
Primers do DNA
/
Chaperonina 10
Idioma:
Inglês
Ano de publicação:
2007
Tipo de documento:
Artigo
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