Ethanol production from Rice (Oryza sativa) straw by simultaneous saccharification and cofermentation.

ABSTRACT

Ethanol production from alkali treated rice straw was investigated by simultaneous saccharification and cofermentation (SSCF) using commercial cellulase and 3 different yeast strains viz., Saccharomyces cerevisiae HAU-1, Pachysolen tannophilus and Candida sp. individually as well as in combination at varied fermentation temperature and incubation time. Dilute alkali (2%) pretreatment of straw resulted in efficient delignification as observed by low residual lignin (12.52%) with 90.6% cellulose and 28.15% hemicellulose recovery. All the 3 yeast strains were able to produce ethanol form alkali treated rice straw and overall ethanol concentration varied from 5.30 to 24.94 g/L based on different fermentation time and temperature. Comparative analysis of ethanol production from different yeast strains combinations revealed maximum ethanol concentration of 23.48 g/L after 96 h incubation at 35ºC with P. tannophilus individually and 24.94 g/L when used as co-culture with Saccharomyces cerevisiae.