Ameliorative effects of MgSO4 on Dexamethasone – induced Histo-chemical alterations in the testes of Albino Rats.

ABSTRACT

Background: In experimental animal, Dexamethasone-induced impaired spermatogenesis, causes distortion of the normal architecture of seminiferous tubules along with alteration in male sexual hormone, testosterone. Concomitant administration of MgSO4 preserved the cytoarchitecture of testes as well as hormonal regulation in albino rats. Objective: This study was performed to observe the ameliorative effects of MgSO4 on the histology of testes and correlation with serum testosterone level during dexamethasone administration. Duration of study twenty days (April 2012).Study design: Prospective experimental study.Place of study Anatomy department Basic Medical Sciences Institute (BMSI), Jinnah Postgraduate Medical Centre (JPMC), Karachi. Methods: thirty healthy adult albino rats were included in this study and divided equally into three groups. Group-A served as control. Group-B received Dexamethasone (intraperitoneally) at the dose of 4.0 mg/kg body weight/24 hours. Group-C received Dexamethasone in the same dose as given in group-B and additionally received MgSO4 (intramuscularly) at the dose of 20mg/kg/24hours. Results: MgSO4 significantly preserved cytoarchitecture of the testes as well as minimized alteration in serum Testosterone level in group-C. Conclusion: This study showed that administration of MgSO4 has ameliorated both the histological and biochemical damaging effects induced by dexamethasone in rats’ testes.