Detection Of Esbl-Producing Escherichia Coli And Klebsiella Pneumoniae In Patient Suffering With Symptomatic Urinary Tract Infection In Tertiary Care Hospital.

ABSTRACT

Aim and objectives: This study is to determine the prevalence of Extended spectrum beta-lactamases (ESBLs) in urinary isolates. To compare different phenotypic methods for ESBL detection and to evaluate the antibiotic resistance patterns among ESBL producing urinary Escherichia coli. Methodology: rd Urinary isolates of E.coli that were resistant to at least one of 3 generation cephalosporins (cefotaxime, ceftazidime) were screened and tested for ESBLproduction using the Double disc diffusion test (DDDT), quantitative E-strip method and Automated Vitek-2. Result: A total of 341 Klebsiella pneumoniae and Escherichia coli isolates were screened from patients with symptomatic UTI. 207 (60.7%) Escherichia coli, 134 (39.2%) Klebsiella pneumonia. ESBL production was detected in 48.8% and for 161 screening test positive E. coli, ESBL production was detected in 40%