A Histomorphometric Evaluation Of Effects Of Sucralose Ingestion On Liver Of Albino Rats

ABSTRACT

Sucralose is a non-nutritive sweetener used in a broad range of foods and beverages. The aim of the study was to demonstrate any histomorphometric changes in liver after sucralose ingestion. Adose of 3g/kg/day of sucralose dissolved in distilled water were given for 30 days to experimental rats by oral gavage whereas Control rats received equal quantity of distilled water. Liver Pieces each having thickness 5mm were taken for paraffin sectioning. 80 slides of 5 micron thick tissue sections were made from each liver and stained with Hematoxylin and Eosin (H & E) which were subsequently evaluated for histomorphometric changes. No significant change in the size of hepatocytes and size of nuclei of hepatocytes were observed between slides of control and sucralose treated rat livers. Sinusoidal width was found to be significantly increased in experimental rat livers as compared to control which is indicative of hepatic damage. A food additive so commonly used as sucralose deserves further investigations.