In vitro Antioxidant and Anti-inflammatory Activities of the Flower Extracts of Hibiscus vitifolius L

ABSTRACT

Objective: To determine the antioxidant and anti-inflammatory activities of the solid powder extracted from the ethyl acetate fraction of the flower Hibiscus vitifolius L. Methods: The flower extract assessed for antioxidant activity using the 1,1–diphenyl-2-picryl-hydrazile (DPPH) radical scavenging assay and the reduced power assay was performed using the Ferric Reducing Capacity (FRC) assay. In vitro anti-inflammatory activity was assessed using human peripheral blood mononuclear cells (PBMC) induced by lipopolysaccharide (LPS) to test the production method of nitric oxide (NO). Results: The solid powder extracted from the ethyl acetate fraction of the flower Hibiscus vitifolius L showed good antioxidant activity in the scavenging DPPH radicals and the FRC assay compared to the standard sample. This powder sample also showed good anti-inflammatory activity in cell viability (LPS induced PBMC) assay and nitric oxide (NO) assay. Conclusion: These results suggest that the powder sample extracted from the ethyl acetate fraction of the flower Hibiscus vitifolius L has substantial antioxidant and anti-inflammatory activity.