Melanocyte spheroids are formed by repetitive long-term trypsinization
Indian J Dermatol Venereol Leprol
;
2019 May; 85(3): 258-265
Artigo
| IMSEAR
| ID: sea-192490
ABSTRACT
Background:
Autologous melanocyte transplantation plays an important role in the treatment of vitiligo.Objective:
Previous studies have indicated that, compared with melanocytes growing in monolayers, melanocyte spheroids have a better survival in growth factor- and serum-deprived conditions.Methods:
Melanocyte spheroids were obtained from human epidermis by repetitive long-term trypsinization and maintained an aggregated morphology for a short period in certain conditions.Results:
Melanocyte spheroids were capable of growing into normal dendritic melanocytes in monolayer when they were harvested and reinoculated in 24-well plates. Immunohistochemical analysis of the melanocyte spheroids revealed that they were positive for HMB45, a melanosome-specific marker. No melanomas occurred when melanocyte spheroids were transplanted into mice.Conclusion:
Our study provides a promising approach for melanocyte transplantation to treat vitiligo.
Texto completo:
DisponíveL
Índice:
IMSEAR (Sudeste Asiático)
Revista:
Indian J Dermatol Venereol Leprol
Ano de publicação:
2019
Tipo de documento:
Artigo
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