A simple medium without blood modified for successful isolation & cultivation of LD bodies.
Artigo
em Inglês
| IMSEAR
| ID: sea-19298
ABSTRACT
A simple growth medium for primary isolation and subsequent cultivation of Leishmania donovani promastigotes without using whole blood is described. This medium is modified from Aljeboori's biphasic medium (used originally only for cultivation), containing only beef extract, peptone, sodium chloride, bactoagar and foetal calf serum (FCS). We have modified the medium by adding glucose and ascertaining the pH in the solid phase and by drastically reducing (91%) FCS in the liquid phase. The medium helps in isolation of L. donovani promastigotes from kala-azar patients, in addition to luxurious growth of parasites. The medium is simple, reliable, reproducible and convenient, with minimal interference in using the parasitic cells for immunological, molecular and isoenzyme studies.
Texto completo:
DisponíveL
Índice:
IMSEAR (Sudeste Asiático)
Assunto principal:
Parasitologia
/
Leishmania donovani
/
Humanos
/
Meios de Cultura Livres de Soro
/
Leishmaniose Visceral
/
Animais
Idioma:
Inglês
Ano de publicação:
1994
Tipo de documento:
Artigo
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