Growth of respiratory syncytial virus in mink lung epithelial cells.
Artigo
em Inglês
| IMSEAR
| ID: sea-19753
ABSTRACT
Mink lung epithelial cells (Mv-1-Lu) were tested for their ability to support the growth and serial passage of respiratory syncytial virus (RSV) in vitro. Indian isolates of RSV induced distinctive cytopathic effect with typical rounding of cells followed by detachment with more than 50 per cent cells showing bright fluorescence using anti-RSV monoclonal antibodies in immunofluorescence test. Serial passage of RSV was possible in Mv-1-Lu cells without loss of sensitivity of the cells for virus growth. Titration of cell associated virus and virus released in the supernatant indicated that 60 per cent of the virus was released in the supernatant, and 40 per cent remained cell associated. Transmission electron microscopic studies of negatively stained RSV particles and ultra-thin sections of RSV infected Mv-1-Lu cells showed roughly spherical particles with club shaped projections, budding from the cytoplasmic membrane. These results indicate that Mv-1-Lu cell line is suitable for the growth and propagation of RSV.
Texto completo:
DisponíveL
Índice:
IMSEAR (Sudeste Asiático)
Assunto principal:
Vírus Sinciciais Respiratórios
/
Cultura de Vírus
/
Feminino
/
Humanos
/
Masculino
/
Linhagem Celular
/
Pré-Escolar
/
Mucosa Respiratória
/
Efeito Citopatogênico Viral
/
Tamanho Celular
Tipo de estudo:
Estudos de avaliação
País/Região como assunto:
Ásia
Idioma:
Inglês
Ano de publicação:
2002
Tipo de documento:
Artigo
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