Transcriptional expression of gastrin mRNA in Helicobacter pylori infected patients.

ABSTRACT

In order to study the association between gastrin and H. pylori infection the density of antral G cells was evaluated by transcriptional expression of gastrin mRNA using a sensitive cold probe labelled with digoxigenin. the study group included 22 patients with symptomatic H. pylori positive gastritis and/or duodenal ulcer, 12 of whom were re-evaluated after eradication of H. pylori and 6 controls. The number of G cells per high power field in H.pylori positive patients (26.68 +/- 9.51) was significantly higher (P < 0.01) compared to controls (5.83 +/- 3.37). Among the 12 patients re-evaluated after H. pylori eradication, there was a significant decrease (P < 0.001) in the number of G cells (13.5 +/- 5.44) compared to pre-eradication value (26.25 +/- 8.0). The present study suggests that increased transcriptional expression of gastrin is directly related to H. pylori infection.