16s rrna gene analysis for characterization of pseudomonas spp. isolated from groundnut rhizosphere

ABSTRACT

The present study assessed 16S rRNA gene sequence analysis method as a tool for identification of fluorescent pseudomonads phenotypically difficult to identify. Using 16S rRNA gene sequencing the isolates were identified to the genus level to the species level. In this study, phylogenetic tree was constructed using complete sequence within the 16S rRNA gene. Distance tree was constructed to find out genetic similarity between the organisms. Although the phylogenetic analysis is not decisive, it is consistent with other observations, especially the capacities of the strain as a biocontrol agent. The results suggest that the JS16, JS7, JS31 and JS52 and strains are high homology with Pseudomonas fluorescens, P. Plecogloccida and P. Putida.