Vancomycin for controlling contamination of selective Kirchner's liquid medium in the culture of gastric lavage for tubercle bacilli.
Artigo
em Inglês
| IMSEAR
| ID: sea-19891
ABSTRACT
Gastric lavage (GL) was collected for culture of tubercle bacilli from children too young to expectorate sputum. The selective Kirchner's liquid medium (SKLM), routinely used as one of the media for culture of all extrapulmonary specimens, was found to often get contaminated when cultured with GL. We have shown that vancomycin at a concentration of 10 mg/l successfully reduced the contamination from about 60 to 20 per cent, and enhanced the isolation rate of tubercle bacilli from 3 to 6 per cent. Decontamination of the liquid culture before subculture on solid medium also helped to reduce the contamination rate. Vancomycin was found to be an effective selective drug for use in the Kirchner's liquid medium for culture of tubercle bacilli.
Texto completo:
DisponíveL
Índice:
IMSEAR (Sudeste Asiático)
Assunto principal:
Humanos
/
Vancomicina
/
Pré-Escolar
/
Técnicas Bacteriológicas
/
Meios de Cultura
/
Lavagem Gástrica
/
Micobactérias não Tuberculosas
Tipo de estudo:
Ensaio Clínico Controlado
Idioma:
Inglês
Ano de publicação:
1995
Tipo de documento:
Artigo
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