Production of Herbal Protein Isolates with the Enzymatic Hydrolysis Technology

ABSTRACT

The article aims to develop the technology of the production of herbal protein isolates with 41% proteinconcentration by enzymatic hydrolysis. The technology includes preparing a hydro module from shredded lupineseeds and water in proportion 110, hydrolysis of starch with alpha-amylase and glucoamylase, centrifugation,autoclaving of the obtained centrifugate at the temperature of 120-130º С and the pressure of 6х105 Pa for 5-6hours, cooling it to the temperature of 36º С and hydrolyzing it with trypsin solution in phosphate buffer solutionat pH 7.5 for 50-60 minutes, centrifugation, heating and drying at the temperature of 95-100 ºС to get the dryresidue concentration of 45% in the protein preparation. Before adding trypsin, it is intensified by blue spectrumlight with the luminous flux of 35 µW/cm2. Based on the research, there are regulated quality indicators of proteinpreparation, storage requirements, and retention periods retention period of 9 months at the temperature of 0-4°С with relative humidity not more than 75%.