Phytochemical Profiling Using Lc-Q-Tof-Ms Analysis And In Vitro Antioxidant Activity Of A Rare Salt-Secreting Mangrove Aegialitis Rotundifolia Roxb. Leaves Extract
Int J Pharm Pharm Sci
;
2019 Mar; 11(3): 37-47
Artigo
| IMSEAR
| ID: sea-205853
ABSTRACT
Objective:
The present work deals with the qualitative study of the phytoconstituents present in Aegialitis rotundifolia Roxb., ethanolic leaves extract and evaluate its antioxidant properties in vitro.Methods:
The qualitative phytochemical analysis of the extract was performed first using preliminary phytochemical tests and then by liquid chromatography quadrupole-time-of-flight mass spectrometry (LC-Q-TOF-MS). The antioxidant properties were investigated comprehensively using seven in vitro models viz., 2, 2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging, nitric oxide (NO) scavenging, hydrogen peroxide (H2O2) scavenging, superoxide (SOD) radical scavenging, lipid peroxidation (LPO) assay, reducing power (RP), and total antioxidant activity.Results:
The preliminary phytochemical analysis revealed the presence of several important phytochemical groups whereas the LC-Q-TOF-MS analysis detected 25 phytoconstituents in the extract mostly belonging to flavonoids and alkaloids. The test extract showed strong dose-dependent antioxidant activity in all the seven in vitro models, however, the activity of the extracts was slightly lower compared to the reference standard ascorbic acid.Conclusion:
The test extract showed strong antioxidant properties which could be possibly due to the phytoconstituents detected in the extract.
Texto completo:
DisponíveL
Índice:
IMSEAR (Sudeste Asiático)
Tipo de estudo:
Pesquisa qualitativa
Revista:
Int J Pharm Pharm Sci
Ano de publicação:
2019
Tipo de documento:
Artigo
Similares
MEDLINE
...
LILACS
LIS