Screening Ethanolic Extract Of Aerva Lanata For Α-Amylase Inhibition And In Vitro Uptake Of Glucose In Adipose Tissue And Psoas Muscle Of Male Sprague Dawley Rats

ABSTRACT

The study was intended to investigate anti-diabetic efficacy of Aerva lanata by determining its α-amylase inhibition activity and in vitro uptake of glucose in adipose tissue and psoas muscle isolated from male Sprague Dawley (SD) rats. Aerva lanata is reported to have many traditional and Ayurvedic uses. Male SD rats (n=3) of 150 g were sacrificed and 250 mg of respective tissues were isolated for the study. Aerva lanata ethanolic extract (ALE) (5-20 mg/mL) showed 13.30 to 54.08% α-amylase inhibition activity. Glucose uptake studies in in vitro conditions were carried out in both adipose tissue and psoas muscle in different sets - tissue alone, tissue along with (Aerva lanata extract 50µg, 100µg, 150µg, insulin 25 mU/L, insulin 50 mU/L and Aerva lanata extract 50µg + insulin 25 mU/L, Aerva lanata extract 100µg + insulin 25 mU/L, Aerva lanata extract 150µg + insulin 25 mU/L, Aerva lanata extract 50µg + insulin 50 mU/L, Aerva lanata extract 100µg + insulin 50 mU/L, Aerva lanata extract 150µg + insulin 50 mU/L). The rate of glucose uptake by insulin action in these tissues was stabilized by ethanolic extract of Aerva lanata and this shows synergetic activity of insulin and Aerva lanata.