Genomic and proteomic approach as a tool to discrimination of Escherichia coli strains of biotechnological interest

ABSTRACT

The Methods based on genomic and proteomic approaches are described as effective tools for the identificationof microorganisms. The development of methodologies capable of differentiating, interspecifically, pathogenic,wild and genetically modified Escherichia coli strains is desirable for the fields of healthcare and Biotechnology.The purpose of this study was to verify the viability of ERIC-PCR and MALDI TOF methods in differentiatinglineages of Escherichia strains. For this purpose, laboratory Escherichia coli ATCC8739, Escherichia coliW3110, Escherichia coli BL21DE3+, Escherichia coli JM109, Escherichia coli MC 1061 and Escherichiacoli DH5α were subjected to ERIC-PCR and MALDI TOF mass spectrometry analyzes. Genomic (ERIC-PCR)and proteomic (MALDI-TOF) methods were able to discriminate between different lineages of Escherichiacoli strains including lineages of Escherichia coli K-12. However, the MALDI TOF proteomic approachrevealed being able to differentiate interspecifically lineages of Escherichia coli strains. The determination ofthe most frequent masses found in the studied Escherichia coli strains in addition to future experiments ofpeptide sequencing profile and SDS-PAGE can be used as a guideline for validating a method for proteomicidentification of these strains.