Detection of SARS-CoV-2 using high-throughput PCR

ABSTRACT

Efficient management of an epidemic requires identification and isolation of infected individuals from healthypopulation to curb community spread of the pathogen. Therefore, a faster and efficient diagnostic methodfor population screening is desirable to flatten the curve of daily raise of cases. This will also help medicalprofessional to treat the epidemic efficiently. The COVID 19 pandemic caused by SARS-CoV-2 coronavirusposes a huge challenge to human being and the countries with high-density population are facing uniquechallenges. For the SARS-CoV-2 pandemic, Quantitative Real Time Polymerase Chain Reaction (qRT-PCR)emerging as a gold standard for the identification of infection; however, infrastructure requirement for thisassay is at a high-demand and therefore, resources are not abundant. We, therefore, propose a Polymerase ChainReaction (PCR)-based sensitive method for the detection of SARS-CoV-2 coronavirus. The pipeline discussedin the paper will broaden the scope of control and surveillance of COVID 19 in a high-throughput mannerwithout a sophisticated diagnostic infrastructure