In vitro antiproliferative effect of aqueous extract of Solanum macranthum fruits on MDA-MB-231 tripple negative breast cancer cell line

ABSTRACT

The current investigation was focused on the assessment of antiproliferative effects of the Solanum macranthumaqueous fruits extract against the human breast cancer cell line MDA-MB-231 in vitro. Phytochemical analysisof the S. macranthum aqueous fruits extract was performed and the phytoconstituents found positive foralkaloids, phenols, and saponins. Quantitative analysis of the total alkaloid and phenol was determined usinggravimetric analysis and folins-phenols reagent method, respectively. The total alkaloid present was estimatedto be 7.58% and total phenol of 158.774 mg/Gallic Acid Equivalent. The cytotoxicity of S. macranthumaqueous fruits extract was screened using 3-(4, 5-dimethylthiazolyl-2)-2, 5-diphenyltetrazolium bromide(MTT) assay and camptothecin was used as standard reference drug. At the concentration of 500 µg/ml, theaqueous extract potentially inhibited the growth of the MDA-MB-231 cell line in vitro reaching 19%, and theIC50 value was calculated to be 525.59 μg/ml. Thus, indicating that aqueous extract of the S. macranthum fruitspossess potential anticancer property.