Partial sequence analysis of a Plasmodium vivax cation transporting ATPase gene homologue & a putative pseudohomologue.
Artigo
em Inglês
| IMSEAR
| ID: sea-21019
ABSTRACT
Molecular characterization of P. vivax is essential to develop suitable antimalarial drugs and vaccines. We describe here isolation and sequence analysis of a partial cDNA of a calcium ATPase as well as a putative pseudogene from this parasite. The immunoscreening of lambda gtll- P. vivax DNA library with patients serum has earlier resulted in the isolation of several seroreactive clones including Pv14. This clone contains a 299 bp insert having 18 amino acids (aa) reading frame fused with beta galactosidase. A larger fragment of approximately 15 kb was isolated from the EMBL3 library for Pv14 but it had only 2 extra aa in its reading frame. The far upstream region of Pv14 revealed a 101aa long putative open reading frame (ORF) showing homology to a variety of calcium ATPases in the M8 and M9 transmembrane region. But in the absence of a transcript in the parasite could indicate that it represents a pseudogene. However, the real gene for calcium ATPase in P. vivax was detected by RT-PCR using degenerate primers, designed from the conserved sequences of energy transduction and phosphorylation domains. The amplified cDNA-PCR product of 550 bp was cloned and sequenced which showed a significant aa homology to the calcium ATPase4 of P. falciparum. The present study, therefore, establishes the existence of calcium ion pumps in P. vivax which will be useful in drug development.
Texto completo:
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Índice:
IMSEAR (Sudeste Asiático)
Assunto principal:
Plasmodium vivax
/
Humanos
/
Dados de Sequência Molecular
/
Sequência de Bases
/
Cálcio
/
Fases de Leitura Aberta
/
Sequência de Aminoácidos
/
Homologia de Sequência de Aminoácidos
/
Transporte de Íons
/
ATPases Transportadoras de Cálcio
Idioma:
Inglês
Ano de publicação:
1998
Tipo de documento:
Artigo
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