Endometrial Tuberculosis Pick By PCR; in Female Infertility

ABSTRACT

"To evaluate the usefulness of PCR in diagnosing genital tuberculosis in female infertility and its sensitivity,specificity, positive predictive value, negative predictive value and its edge over other diagnostic modalitieslike histopathological examination and culture. This study included 100 infertile women who met the inclusionand exclusion criteria. After a detailed history, clinical examination and investigations, endometrial curettingswere taken in premenstrual phase and sent for culture, histopathological examination and PCR. Afterevaluation the results were compared individually with each other or in combination to see the positive pickup percentage and their sensitivity and specificity. We found 4% cases positive for tuberculosis on culturefor Mycobacterium tuberculosis, 7% of cases positive histopathologicaly and 15% cases on PCR evaluation.The sensitivity for diagnosing this disease was found highest in PCR (100%) followed by histopathologicalexamination (75%). Culture had the least sensitivity of 42.85%. PCR represents a rapid and sensitivemethod for identification of Mycobacterium DNA in female genital tuberculosis. Culture and histopathologyhave low sensitivity compared to PCR. Therefore, when the clinical suspicion is high and culture andhistopathology is negative, PCR is the method of choice for identifying the infection."