Uterine Fibroid: Isolation, Culture and Characterization

ABSTRACT

Present study was carried out to establish uterine fibroid primary culturesystem for screening of natural/synthetic compounds against uterine fibroid. For invitro culture, enzymatic isolation method was used. To characterize, histochemistry (H& E, Masson’s Trichrome and Periodic Acid Schiff) staining and immunocytochemistryusing marker antibodies (Versican) were performed in vitro. Uterine fibroid tissueshowed much intense staining of Masson’s Trichrome and Periodic Acid Schiff stain ascompared to adjacent myometrium tissue. The primary cultured cells showedsignificantly higher proliferation, sub-culture efficiency and expression of Versicanprotein. In conclusion, our results suggest that in vitro cultured uterine fibroid cells mayoffer a suitable alternative model to evaluate natural or synthetic compounds havingantitumor properties for uterine fibroid treatment.