Development & use of antichlamydial monoclonal antibodies for rapid diagnosis of follicular conjunctivitis.
Artigo
em Inglês
| IMSEAR
| ID: sea-21562
ABSTRACT
We developed four independent murine hybrid clones producing IgG class and genus specific monoclonal antibodies against the major outer membrane protein (MOMP) of Chlamydia trachomatis, L2 serovar. All antibodies reacted with a single epitope of MOMP. In indirect immunofluorescence assay using one of the antibodies chlamydiae could be detected in 12 of 23 conjunctival scrapings from patients of follicular conjunctivitis. Giemsa staining could detect classical inclusion bodies in only 5 of these 23 smears. Antigen detection using these monoclonal antibodies may be used as a rapid diagnostic test in clinical and epidemiological screening for chlamydial infection.
Texto completo:
DisponíveL
Índice:
IMSEAR (Sudeste Asiático)
Assunto principal:
Fatores de Tempo
/
Chlamydia trachomatis
/
Conjuntivite Bacteriana
/
Animais
/
Camundongos
/
Anticorpos Monoclonais
Idioma:
Inglês
Ano de publicação:
1994
Tipo de documento:
Artigo
Similares
MEDLINE
...
LILACS
LIS