Analysis of the major surfactant-associated proteins in human lung.
Artigo
em Inglês
| IMSEAR
| ID: sea-23699
ABSTRACT
The protein component of human pulmonary surfactant was analysed. A purified surfactant preparation, after delipidation, was subjected to gel filtration on Sephadex G-200. The proteins present in the surfactant were categorised by means of SDS-polyacrylamide gel electrophoresis into serum and non-serum components. Molecular masses determination showed the presence of three sub-groups with molecular masses of 60-68 kDa, 28-36 kDa and 10-18 kDa, respectively. Antiserum generated against 28-36 kDa protein strongly reacted with the purified surfactant and amniotic fluid, while it did not show any cross reactivity with other groups of proteins and serum in a double diffusion immunoprecipitation assay. We propose that this protein is the major non-serum surfactant-associated protein present in human lung surfactant.
Texto completo:
DisponíveL
Índice:
IMSEAR (Sudeste Asiático)
Assunto principal:
Surfactantes Pulmonares
/
Humanos
/
Testes de Precipitina
/
Proteínas Sanguíneas
/
Cromatografia em Gel
/
Adulto
/
Eletroforese em Gel de Poliacrilamida
/
Soros Imunes
/
Pulmão
/
Peso Molecular
Idioma:
Inglês
Ano de publicação:
1992
Tipo de documento:
Artigo
Similares
MEDLINE
...
LILACS
LIS