Resolution of DL-Phenylglycine by Penicillin G acylase.
Hindustan Antibiot Bull
;
2005-2006; 47-48(): 41-4
Artigo
em Inglês
| IMSEAR
| ID: sea-2375
ABSTRACT
The parameters for complete hydrolysis of L-phenyl acetyl phenylglycine (L-PAPG) using immobilized penicillin G acylase (IMEPGA) were investigated. IMEPGA exhibited maximum activity at pH 8.5 and 50 degrees C. The apparent Km value observed was 10 mM. Quantitative hydrolysis (>97%) of the L-PAPG was achieved within 45 min, at pH 7.8 and 37 degrees C, when 0.5% (w/v) of DL-PAPG was used and the concentration of IMEPGA was 133 IU/gm of DL-PAPG. The IMEPGA was used for 50 cycles.
Texto completo:
DisponíveL
Índice:
IMSEAR (Sudeste Asiático)
Assunto principal:
Penicilina Amidase
/
Polímeros
/
Temperatura
/
Enzimas Imobilizadas
/
Glicina
/
Concentração de Íons de Hidrogênio
Idioma:
Inglês
Revista:
Hindustan Antibiot Bull
Ano de publicação:
2005
Tipo de documento:
Artigo
Similares
MEDLINE
...
LILACS
LIS