Enzymatic hydrolysis of 7-phenylacetamidodesacetoxycephalosporanic acid by immobilized penicillin G acylase.
Hindustan Antibiot Bull
;
1989 Aug-Nov; 31(3-4): 79-82
Artigo
em Inglês
| IMSEAR
| ID: sea-2423
ABSTRACT
Enzymatic parameters such as pH, temperature and substrate concentration were studied for the hydrolysis of 7-PADCA by penicillin G acylase. Optimum pH and temperature were 8.0 and 50 degrees C, respectively. Km value of soluble and immobilized enzyme for 7-PADCA was 2.3 x 10(-5) M and 7.5 x 10(-5) M, respectively. At 7-PADCA concentration of 5% and an IME 7-PADCA ratio of 12.5, the hydrolysis was complete in 110 min.
Texto completo:
DisponíveL
Índice:
IMSEAR (Sudeste Asiático)
Assunto principal:
Penicilina Amidase
/
Temperatura
/
Cefalosporinas
/
Enzimas Imobilizadas
/
Concentração de Íons de Hidrogênio
/
Hidrólise
Idioma:
Inglês
Revista:
Hindustan Antibiot Bull
Ano de publicação:
1989
Tipo de documento:
Artigo
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