Elevated percentage of perforin positive cells in active pulmonary tuberculosis.
Artigo
em Inglês
| IMSEAR
| ID: sea-24354
ABSTRACT
BACKGROUND & OBJECTIVES:
Perforin is one of the major effector molecules of cytotoxic cells associated with killing of cells harbouring intracellular bacterial infection. The precise role of perforin positive cells in tuberculosis still remains controversial. The present study was done to determine the number of circulating CD4(+) and CD8(+) perforin positive cells to assess the level of cytotoxic response against Mycobacterium tuberculosis in patients with pulmonary tuberculosis.METHODS:
Intracellular perforin and surface CD4 and CD8 staining of peripheral blood lymphocytes was done using specific monoclonal antibodies and enumerated using flowcytometry.RESULTS:
A significantly decreased total lymphocytes (P<0.01), CD4 (P<0.001) and CD8 (P<0.01) lymphocyte counts in PTB patients was observed compared to normal healthy individuals (NHS). Intracellular perforin staining showed significantly elevated percentages of total (P<0.05) and CD8 (P<0.01) perforin positive cells in PTB patients compared to NHS. However, the absolute counts of total, CD4 and CD8 cells positive for perforin were similar in patients and NHS. INTERPRETATION &CONCLUSION:
Our results suggest that during active stage of pulmonary tuberculosis there was an increased percentage of CD8 cells positive for perforin, irrespective of their absolute counts. Further, CD8(+) perforin positive cells may have increased cytolytic activity against M. tuberculosis in active pulmonary tuberculosis.
Texto completo:
DisponíveL
Índice:
IMSEAR (Sudeste Asiático)
Assunto principal:
Tuberculose Pulmonar
/
Humanos
/
Glicoproteínas de Membrana
/
Linfócitos T CD4-Positivos
/
Estudos de Casos e Controles
/
Linfócitos T CD8-Positivos
/
Adulto
/
Proteínas Citotóxicas Formadoras de Poros
/
Perforina
/
Imunidade Celular
Tipo de estudo:
Estudo observacional
/
Fatores de risco
Idioma:
Inglês
Ano de publicação:
2006
Tipo de documento:
Artigo
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