Effect of heat-shock & nutritional stress on the expression of a neutral thiol protease in Leishmania donovani promastigotes.
Artigo
em Inglês
| IMSEAR
| ID: sea-25724
ABSTRACT
The soluble intracellular protease was partially purified from L. donovani promastigotes. The activity of this enzyme increased with increase in temperature from 25 degrees C to 37 degrees C and was active optimally at 70 degrees C. This protease activity appeared to be decreased due to heat-shock of the promastigotes for 4 h at 37 degrees C and increased due to nutrient starvation. Inhibition of the protease by p-chloromercuribenzoate and iodoacetamide suggested that this enzyme could be a thiol protease.
Texto completo:
DisponíveL
Índice:
IMSEAR (Sudeste Asiático)
Assunto principal:
Leishmania donovani
/
Humanos
/
Cisteína Endopeptidases
/
Meios de Cultura
/
Temperatura Alta
/
Animais
Idioma:
Inglês
Ano de publicação:
1989
Tipo de documento:
Artigo
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