Barley oxalate oxidase immobilized on zirconia-coated alkylamine glass using glutaraldehyde.
Indian J Biochem Biophys
;
1993 Feb; 30(1): 54-7
Artigo
em Inglês
| IMSEAR
| ID: sea-26239
ABSTRACT
A method for immobilizing barley oxalate oxidase to zirconia coated alkylamine glass through the process of glutaraldehyde coupling has been described. The immobilized enzyme retained 97.2% of the specific activity, with a conjugation yield of 6.63 mg/g support and showed an increase in optimum pH. The Km value of immobilized enzyme was unaltered but Vmax was decreased compared to free enzyme. The conjugated enzyme was stable at 4 degrees C for 2 years. A number of inorganic ions and metabolic substances did not denature the immobilized enzyme. The clinical importance of this work is demonstrated.
Texto completo:
DisponíveL
Índice:
IMSEAR (Sudeste Asiático)
Assunto principal:
Oxirredutases
/
Plantas
/
Hordeum
/
Zircônio
/
Estabilidade Enzimática
/
Glutaral
/
Enzimas Imobilizadas
/
Aminas
/
Concentração de Íons de Hidrogênio
Idioma:
Inglês
Revista:
Indian J Biochem Biophys
Ano de publicação:
1993
Tipo de documento:
Artigo
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