Construction, purification and characterization of a recombinant baculovirus containing the gene for alpha subunit of human chorionic gonadotropin.
Indian J Biochem Biophys
;
1991 Aug; 28(4): 237-42
Artigo
em Inglês
| IMSEAR
| ID: sea-26280
ABSTRACT
A cDNA encoding the alpha subunit of human chorionic gonadotropin, a placental glycoprotein hormone, was cloned downstream to the viral polyhedrin gene promoter of Autographa california nuclear polyhedrosis virus and the recombinant transfer vector was used to co-transfect Spodoptera frugiperda cells growing in culture. Recombinant baculovirus carrying the alpha hCG gene was detected and isolated after dot hybridization using supernatant from co-transfected cells. Recombinant vAc alpha hCG having a replacement of the viral polyhedrin gene, which is hyper-transcribed very late in the infection cycle, with the alpha hCG cDNA was purified after a single round of plaque purification. Insect cell culture infected with vAc alpha hCG, secreted high levels of hCG which was biologically active.
Texto completo:
DisponíveL
Índice:
IMSEAR (Sudeste Asiático)
Assunto principal:
Humanos
/
DNA
/
Subunidade alfa de Hormônios Glicoproteicos
/
Mapeamento por Restrição
/
Baculoviridae
/
Clonagem Molecular
/
Vetores Genéticos
Idioma:
Inglês
Revista:
Indian J Biochem Biophys
Ano de publicação:
1991
Tipo de documento:
Artigo
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