Role of positive charge of lysine residue on ribosome-inactivating property of gelonin.
Indian J Biochem Biophys
;
2001 Oct; 38(5): 309-12
Artigo
em Inglês
| IMSEAR
| ID: sea-26321
ABSTRACT
The report that gelonin cross-linked with monoclonal antibodies with the use of 2-iminothiolane (2-IT) exhibited higher cytotoxicity than the conjugates prepared with the use of N-succinimidyl-3-(2-pyridylthio) propionate (SPDP) alone, has prompted us to investigate the effect of epsilon-NH2 group modification with 2-IT on the ribosome-inactivating property (RIP) of gelonin. The purified gelonin was modified with 2-IT at a different molar ratio and their effects on immunoreactivity and ribosome-inactivating property were compared with those of N-succinimidyl 6-[3-(2-pyridyldithio) propionamido] hexanoate (long chain-SPDP) and SPDP modified gelonin derivatives. Modification of single amino group with 2-IT results in about 25-50% inhibition of immunoreactivity and 60-70% loss of protein synthesis inhibition activity. Modification of 2-3 amino groups further hampers both immunoreactivity and protein synthesis inhibition property of gelonin. Both the long chain-SPDP with SPDP modifications showed more pronounced effects on immunoreactivity and RIP activity as compared to the similar ratio of 2-IT modification(s). It may, therefore, be concluded that the positive charge plays an important role in the immunological as well as the protein synthesis inhibitory effect of gelonin.
Texto completo:
DisponíveL
Índice:
IMSEAR (Sudeste Asiático)
Assunto principal:
Proteínas de Plantas
/
Ribossomos
/
Succinimidas
/
Inibidores da Síntese de Proteínas
/
Sistema Livre de Células
/
Reagentes de Ligações Cruzadas
/
Desoxirribonucleases
/
Proteínas Inativadoras de Ribossomos Tipo 1
/
Imidoésteres
/
Lisina
Idioma:
Inglês
Revista:
Indian J Biochem Biophys
Ano de publicação:
2001
Tipo de documento:
Artigo
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