Purification of a peroxidase from Solanum melongena fruit juice.
Indian J Biochem Biophys
;
2006 Aug; 43(4): 239-43
Artigo
em Inglês
| IMSEAR
| ID: sea-26352
ABSTRACT
Solanum melongena fruit juice contains peroxidase activity of the order of 0.125 IU/mL. A method for the 11-fold purification of the enzyme was developed. The Km values of the peroxidase for the substrates guaiacol and hydrogen peroxide were 6.5 mM and 0.33 mM, respectively. The pH and temperature optima were 5.5 and 84 degrees C, respectively using guaiacol as the substrate. Sodium azide and phenyl hydrazine inhibited the enzyme competitively.
Texto completo:
DisponíveL
Índice:
IMSEAR (Sudeste Asiático)
Assunto principal:
Peroxidases
/
Fenil-Hidrazinas
/
Temperatura
/
Bebidas
/
Cinética
/
Azida Sódica
/
Solanum melongena
/
Relação Dose-Resposta a Droga
/
Frutas
/
Guaiacol
Idioma:
Inglês
Revista:
Indian J Biochem Biophys
Ano de publicação:
2006
Tipo de documento:
Artigo
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