Rat brain membrane-bound delta opioid receptor: loss and reactivation of binding on dialysis and aging at low temperature.
Indian J Biochem Biophys
; 2001 Jun; 38(3): 166-9
Article
em En
| IMSEAR
| ID: sea-26510
ABSTRACT
A change in the environment of rat brain membranes by dialysis from phosphate buffered saline (PBS) to 10 mM potassium phosphate (pH 7.2) led to a 35% loss in delta opioid receptor binding, while alteration of membrane structure on freezing at -20 degrees C for 55 days led to 85% loss of receptor binding. The dialysate, 200 mM KCI and NaCl restored receptor binding lost on dialysis. This K+ and Na+ restabilization of the receptor can be through cation-pi bonding, interactions that are suited to the lipid bilayer. In membranes stored at -20 degrees C, the loss of binding is attributed to increased membrane fluidity by phospholipase A2 action on membrane phospholipids, resulting in an increase of free fatty acids. K+ but not Na+ restabilization of these membrane receptors may be due to the ability of K+ to decrease membrane fluidity.
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Assunto principal:
Fosfolipases A
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Fosfolipídeos
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Potássio
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Ligação Proteica
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Ratos
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Sódio
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Temperatura
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Fatores de Tempo
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Encéfalo
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Membrana Celular
Idioma:
En
Revista:
Indian J Biochem Biophys
Ano de publicação:
2001
Tipo de documento:
Article