Increase in alpha-glycerophosphate dehydrogenase and other oxidoreductase activities of hepatic mitochondria on administration of vanadate to the rat.
Indian J Biochem Biophys
;
1989 Aug; 26(4): 227-33
Artigo
em Inglês
| IMSEAR
| ID: sea-26597
ABSTRACT
Liver mitochondria isolated from vanadate-administered rats showed increased (20-25%) rates of oxidation of both NAD(+)-linked substrates and succinate. Respiratory control index and ADP/O were unaffected by the treatment. Dormant and uncoupler-stimulated ATPase activity also was not affected by vanadate administration. Membrane-bound, electron-transport-linked dehydrogenase activities (both NAD(+)- and succinate-dependent) increased by 15-20% on vanadate treatment. Mitochondrial alpha-glycerophosphate dehydrogenase activity increased by 50% on vanadate administration. The above effects of vanadate on oxidoreductase activities could be prevented by the prior administration of antagonists to alpha-adrenergic receptors. Substrate-dependent H2O2 generation by mitochondria also showed an increase on vanadate administration.
Texto completo:
DisponíveL
Índice:
IMSEAR (Sudeste Asiático)
Assunto principal:
Fosforilação Oxidativa
/
Oxirredutases
/
Ratos
/
Ratos Endogâmicos
/
Masculino
/
Mitocôndrias Hepáticas
/
Vanadatos
/
Peróxido de Hidrogênio
/
Animais
Idioma:
Inglês
Revista:
Indian J Biochem Biophys
Ano de publicação:
1989
Tipo de documento:
Artigo
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