Reconstitution of denatured E. coli alkaline phosphatase with E. coli ribosome.
Indian J Biochem Biophys
;
1992 Dec; 29(6): 512-5
Artigo
em Inglês
| IMSEAR
| ID: sea-26610
ABSTRACT
E. coli alkaline phosphatase was denatured by physical/chemical means. In vitro reconstitution of this denatured enzyme was assisted by 70S E. coli ribosome, as shown by the recovery of its catalytic competence. Almost total recovery of activity of the totally inactivated enzyme was obtained in presence of equimolar concentration of 70S ribosome at 50 degrees C.
Texto completo:
DisponíveL
Índice:
IMSEAR (Sudeste Asiático)
Assunto principal:
Desnaturação Proteica
/
Ribossomos
/
Termodinâmica
/
Cinética
/
Dobramento de Proteína
/
Fosfatase Alcalina
/
Escherichia coli
Idioma:
Inglês
Revista:
Indian J Biochem Biophys
Ano de publicação:
1992
Tipo de documento:
Artigo
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