Rat liver chromatin digested in situ with endogeneous Mg(2+)-dependent deoxyribonuclease exhibits two distinct DNA repeats.
Indian J Biochem Biophys
;
1992 Feb; 29(1): 42-8
Artigo
em Inglês
| IMSEAR
| ID: sea-26625
ABSTRACT
Incubation of rat liver nuclei in the presence of 1.0-5.0 mM Mg2+ at 37 degrees C releases oligonucleosomes containing at least two distinct chromatin-DNA repeat elements. The 'short' repeat is derived from the dimer to pentamer series, while the 'long' repeat is found in the monomer and hexamer to decanucleosomes. Both repeat lengths decrease during enzymatic hydrolysis but in 5.0 mM Mg2+, which is optimal concentration, the 'long' repeat is degraded faster.
Texto completo:
DisponíveL
Índice:
IMSEAR (Sudeste Asiático)
Assunto principal:
Ratos
/
DNA
/
Cromatina
/
Sequências Repetitivas de Ácido Nucleico
/
Endodesoxirribonucleases
/
Fígado
/
Animais
/
Magnésio
Idioma:
Inglês
Revista:
Indian J Biochem Biophys
Ano de publicação:
1992
Tipo de documento:
Artigo
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