Determination of serum glucose using co-immobilized glucose oxidase and peroxidase onto arylamine glass beads affixed on a plastic strip.
Indian J Biochem Biophys
;
2005 Dec; 42(6): 391-4
Artigo
em Inglês
| IMSEAR
| ID: sea-26751
ABSTRACT
Glucose oxidase (GOD) from Aspergillus niger and horseradish peroxidase (POD) were co-immobilized onto arylamine glass beads affixed on a plastic strip with a conjugation yield of 28.2 mg/g and 43% retention of their initial specific activity. The coimmobilized enzymes showed maximum activity at pH 7.5 when incubated at 37 degrees C for 15 min. A simple, specific and sensitive method for discrete analysis of the serum glucose was developed employing this strip. The minimum detection limit of the method was 5 mg/dl. Within and between assay coefficient of variations for the serum were <5.6% and <10.6% (n = 6) respondely. A good correlation (r = 0.943) was found between the glucose values obtained by the enzyme colorimetric method employing free GOD and POD and the present method. The strip lost 50% of its initial activity after its 150 regular uses for a period of one month, when stored in reaction buffer at 4 degrees C. The method is cost-effective than the enzymic colorimetric method, as the enzyme strip is reusable.
Texto completo:
DisponíveL
Índice:
IMSEAR (Sudeste Asiático)
Assunto principal:
Plásticos
/
Aspergillus niger
/
Glicemia
/
Feminino
/
Humanos
/
Masculino
/
Adulto
/
Diabetes Mellitus
/
Enzimas Imobilizadas
/
Aminas
Idioma:
Inglês
Revista:
Indian J Biochem Biophys
Ano de publicação:
2005
Tipo de documento:
Artigo
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