Alterations in brain tumor DNA detected by a fingerprinting probe.
Indian J Biochem Biophys
;
1996 Dec; 33(6): 455-7
Artigo
em Inglês
| IMSEAR
| ID: sea-26769
ABSTRACT
We used a novel DNA fingerprinting probe O-chi-1 (ref. 1) to detect differences in the hybridization pattern of brain tumor DNA and paired normal tissue of a given individual. Representatives of meningiomas (two), glioblastoma multeforme (three) and astrocytoma (one) were studied. Alterations, which included amplification as well as the loss of a normal band in tumor DNA, were observed in four of the six tumours. While the increased intensity of a band can be taken to imply increased copy number, the disappearance of bands could either be due to loss of DNA sequence or rearrangement resulting in different sized bands.
Texto completo:
DisponíveL
Índice:
IMSEAR (Sudeste Asiático)
Assunto principal:
Astrocitoma
/
Neoplasias Encefálicas
/
Humanos
/
DNA de Neoplasias
/
Desoxirribonucleases de Sítio Específico do Tipo II
/
Sondas de DNA
/
Southern Blotting
/
Impressões Digitais de DNA
/
Glioblastoma
/
Eletroforese em Gel de Ágar
Idioma:
Inglês
Revista:
Indian J Biochem Biophys
Ano de publicação:
1996
Tipo de documento:
Artigo
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