Isolation of stress responsive Psb A gene from rice (Oryza sativa l.) using differential display.
Indian J Biochem Biophys
;
2006 Aug; 43(4): 244-6
Artigo
em Inglês
| IMSEAR
| ID: sea-26910
ABSTRACT
Differential display (DD) experiments were performed on drought-tolerant rice (Oryza sativa L.) genotype N22 to identify both upregulated and downregulated partial cDNAs with respect to moisture stress. DNA polymorphism was detected between drought-stressed and control leaf tissues on the DD gels. A partial cDNA showing differential expression, with respect to moisture stress was isolated from the gel. Northern blotting analysis was performed using this cDNA as a probe and it was observed that mRNA corresponding to this transcript was accumulated to high level in rice leaves under water deficit stress. At the DNA sequence level, the partial cDNA showed homology with psb A gene encoding for Dl protein.
Texto completo:
DisponíveL
Índice:
IMSEAR (Sudeste Asiático)
Assunto principal:
Polimorfismo Genético
/
Oryza
/
Bioquímica
/
Dados de Sequência Molecular
/
Sequência de Bases
/
Cloroplastos
/
Northern Blotting
/
Genes de Plantas
/
DNA Complementar
/
DNA de Plantas
Idioma:
Inglês
Revista:
Indian J Biochem Biophys
Ano de publicação:
2006
Tipo de documento:
Artigo
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