Biochemical activities of denatured covalently closed circular duplex DNA.
Indian J Biochem Biophys
;
1993 Apr; 30(2): 123-7
Artigo
em Inglês
| IMSEAR
| ID: sea-27108
ABSTRACT
Covalently closed circular duplex DNA when exposed to high alkaline pH followed by neutralization yields a collapsed state structure (form I(d)) that can undergo transition to form I and was susceptible to S1 nuclease. Form I(d), in spite of its compact structure, admits specific cleavage by restriction enzymes over its entire genome. When used in a semi-in vitro replication complex, form I(d) gave significantly better template activity, and undergoes better primer extension in in vitro using Klenow. Thus form I(d) having a compact shape can behave as a better substrate in a few key enzymatic reactions.
Texto completo:
DisponíveL
Índice:
IMSEAR (Sudeste Asiático)
Assunto principal:
Plantas
/
DNA Circular
/
Estrutura Molecular
/
Replicação do DNA
/
Concentração de Íons de Hidrogênio
/
Desnaturação de Ácido Nucleico
Idioma:
Inglês
Revista:
Indian J Biochem Biophys
Ano de publicação:
1993
Tipo de documento:
Artigo
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