In vitro characterization of repair synthesis initiated by T4 endonuclease V on a synthetic DNA substrate.
Indian J Biochem Biophys
;
1992 Jun; 29(3): 227-30
Artigo
em Inglês
| IMSEAR
| ID: sea-27303
ABSTRACT
The size of the repair patch produced by E. coli DNA polymerase (Pol I) following the removal of a pyrimidine dimer from DNA in response to the nicking activity of T4 endonuclease (T4 endo V) was determined. A 48-bp DNA containing a pyrimidine dimer at a defined location was labelled in the damaged strand and incubated with T4 endo V and E. coli endonuclease IV. Subsequently, DNA synthesis by DNA Pol I was carried out in the presence of four dNTPs, ATP and DNA ligase. Analysis of the reaction products on a sequencing gel revealed a ladder of only 4-oligonucleotides, 1-4 nucleotides greater in length than the fragment generated by the combined nicking activities of T4 endo V and E. coli endonuclease IV. Thus we conclude that the in vitro repair patch size of T4 endo V is 4 nucleotides and that in some cases the repaired DNA is not ligated.
Texto completo:
DisponíveL
Índice:
IMSEAR (Sudeste Asiático)
Assunto principal:
Oligodesoxirribonucleotídeos
/
Especificidade por Substrato
/
Fagos T
/
Proteínas Virais
/
DNA
/
Dados de Sequência Molecular
/
Sequência de Bases
/
Desoxirribonuclease (Dímero de Pirimidina)
/
DNA Polimerase I
/
Reparo do DNA
Idioma:
Inglês
Revista:
Indian J Biochem Biophys
Ano de publicação:
1992
Tipo de documento:
Artigo
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