Cloned single copy DNA sequences of Mycobacterium tuberculosis as DNA probes.
Indian J Biochem Biophys
;
1991 Aug; 28(4): 243-6
Artigo
em Inglês
| IMSEAR
| ID: sea-27305
ABSTRACT
A recombinant genomic clone was isolated from a lambda gt 11 library of M. tuberculosis on the basis of lack of hybridization with M. avium and M. kansasi. The specificity and sensitivity of M. tb DNA probes, 2.5 and 2.3 kb in size, were assessed by Southern blot and dot blot hybridization. These did not cross hybridize to DNA of mycobacteria other than members of M. tb complex, nor with DNA of non mycobacterial origin. Sensitivity was determined to be 200 pg which is equivalent to 10(4) bacilli. Genomic Southern hybridization indicated single copy nature of the probes.
Texto completo:
DisponíveL
Índice:
IMSEAR (Sudeste Asiático)
Assunto principal:
Tuberculose
/
Humanos
/
DNA Bacteriano
/
Sondas de DNA
/
Clonagem Molecular
/
Mycobacterium tuberculosis
/
Hibridização de Ácido Nucleico
Idioma:
Inglês
Revista:
Indian J Biochem Biophys
Ano de publicação:
1991
Tipo de documento:
Artigo
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