Polyclonal antibodies as probes to distinguish between tight and loose couple 50S ribosomes of Escherichia coli.
Indian J Biochem Biophys
;
1991 Oct-Dec; 28(5-6): 369-73
Artigo
em Inglês
| IMSEAR
| ID: sea-27327
ABSTRACT
Antibody has been raised in rabbit against L7/L12 protein of E. coli 50S ribosomes and purified, finally through affinity column. A sensitive assay method using ELISA technique has also been standardised. LC 50S ribosomes react more with the antibody than TC 50S ribosomes. This supports the earlier physical data [Burma D P, Srivastava A K, Srivastava S, Tewari D S, Dash D & Sengupta S K, (1984), Biochem Biophys Res Commun, 124, 970] indicating that L7/L12 stalk region is protruded in medium in LC ribosomes and folded towards the body in TC ribosomes.
Texto completo:
DisponíveL
Índice:
IMSEAR (Sudeste Asiático)
Assunto principal:
Proteínas Ribossômicas
/
Ribossomos
/
Sondas Moleculares
/
Proteínas de Escherichia coli
/
Escherichia coli
/
Anticorpos Antibacterianos
Idioma:
Inglês
Revista:
Indian J Biochem Biophys
Ano de publicação:
1991
Tipo de documento:
Artigo
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