Developmentally-regulated cell surface N-linked oligosaccharides participate in intercellular cohesion in Dictyostelium discoideum.
Indian J Biochem Biophys
;
1993 Dec; 30(6): 376-81
Artigo
em Inglês
| IMSEAR
| ID: sea-27343
ABSTRACT
The ability of purified plasma membrane glycoconjugates to inhibit the EDTA-resistant agglutination between aggregation-stage cells of Dictyostelium discoideum has suggested that receptor binding of these glycoconjugates provides a basis for cell-cell cohesion during aggregation. This has been tested by analysis of a series of mutants with different defects in the assembly of N-linked oligosaccharides. Mutant HL241 lacks outer branch components of N-linked oligosaccharides and fails to aggregate or express EDTA-resistant cohesion. HL244 makes unsulphated but otherwise normal N-linked oligosaccharides, generates multiple tips on aggregated cell mounds in some clones, and shows abnormally strong EDTA-resistant cohesion. Two mutants that are temperature-sensitive for complete processing of N-linked oligosaccharides are also temperature-sensitive for expression of both aggregation ability and EDTA-resistant cohesion. A revertant that recovered essentially normal N-linked oligosaccharide processing at the restrictive temperature has also recovered its ability to aggregate and to agglutinate in EDTA.
Texto completo:
DisponíveL
Índice:
IMSEAR (Sudeste Asiático)
Assunto principal:
Oligossacarídeos
/
Glicoproteínas de Membrana
/
Dados de Sequência Molecular
/
Glicolipídeos
/
Membrana Celular
/
Sequência de Carboidratos
/
Aglutinação
/
Dictyostelium
/
Animais
/
Lipídeos de Membrana
Idioma:
Inglês
Revista:
Indian J Biochem Biophys
Ano de publicação:
1993
Tipo de documento:
Artigo
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