Development of a 23S rRNA-based PCR assay for the detection of mycobacteria.
Indian J Biochem Biophys
;
1994 Aug; 31(4): 288-94
Artigo
em Inglês
| IMSEAR
| ID: sea-27463
ABSTRACT
The partial nucleotide sequence of a recombinant plasmid containing the 23S rRNA gene of Mycobacterium tuberculosis was determined and an assay was developed for amplifying 23S rRNA gene sequences of mycobacteria. The PCR-based non-radioactive test enabled us to distinguish Mycobacterium from other closely related genera and was sensitive enough to detect 2 bacterial genome equivalents. The assay was extended to the detection of mycobacterial DNA in uncultured clinical specimens; 23S rRNA sequences were detected in thirty four of forty eight (70.8%) sputum and cerebrospinal fluid (CSF) specimens by the PCR assay, whereas direct smear examination and culture methods demonstrated a positivity rate of 29.2% and 16.7% respectively for the same specimens. A RNA-based PCR assay with a detection limit of 1 genome equivalent was also developed. These PCR assays should prove useful for the early and rapid detection of mycobacterial infection in uncultured clinical specimens.
Texto completo:
DisponíveL
Índice:
IMSEAR (Sudeste Asiático)
Assunto principal:
Humanos
/
Dados de Sequência Molecular
/
RNA Ribossômico 23S
/
Sequência de Bases
/
Reação em Cadeia da Polimerase
/
Sensibilidade e Especificidade
/
Mycobacterium tuberculosis
Tipo de estudo:
Estudo diagnóstico
Idioma:
Inglês
Revista:
Indian J Biochem Biophys
Ano de publicação:
1994
Tipo de documento:
Artigo
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