Comparative analysis of surface components of adult, micro-filariae and infective larvae of Brugia malayi.
Indian J Biochem Biophys
;
1989 Feb; 26(1): 52-5
Artigo
em Inglês
| IMSEAR
| ID: sea-27489
ABSTRACT
A comparative analysis of surface proteins of adult, microfilariae and infective larvae of Brugia malayi, the human filarial parasite, has been carried out using IODOGEN (1,3,4,6-tetrachloro-3,alpha 6 alpha-diphenyl-glycoluril) and lactoperoxidase methods. SDS-polyacrylamide gel electrophoretic and autoradiographic analyses revealed the presence of 9 proteins (15-200 kDa) in adults, while microfilariae and infective larvae showed 8 and 6 proteins (15-120 kDa), respectively. The pattern of proteins radiolabelled by IODOGEN method was very similar to that of proteins labelled by the lactoperoxidase method. Since these proteins are released by the protease treatment of whole parasites, they are likely to be present on the surface of the parasite.
Texto completo:
DisponíveL
Índice:
IMSEAR (Sudeste Asiático)
Assunto principal:
Ureia
/
Brugia
/
Radioisótopos do Iodo
/
Lactoperoxidase
/
Larva
/
Animais
/
Proteínas de Membrana
/
Microfilárias
Idioma:
Inglês
Revista:
Indian J Biochem Biophys
Ano de publicação:
1989
Tipo de documento:
Artigo
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