Is asparagine-linked protein glycosylation an obligatory requirement for angiogenesis?
Artigo
em Inglês
| IMSEAR
| ID: sea-27515
ABSTRACT
Dependence of protein N-glycosylation on capillary endothelial cell proliferation has been studied. Amphomycin, a potent N-glycosylation inhibitor, inhibited capillary endothelial cell proliferation in a dose-dependent manner. beta-Agonist isoproterenol as well as other intracellular cAMP enhancing agents, viz. cholera toxin, prostaglandin E1 and 8Br-cAMP, also enhanced capillary endothelial cell proliferation. In addition to cell proliferation, isoproterenol also enhanced protein glycosylation in these cells. Isoproterenol effect was mediated by beta-adrenoreceptors, as it got reduced on pre-treatment of cells with either atenolol or ICI 118, 551 or propranolol. Furthermore, isoproterenol stimulation of protein glycosylation by exogenous dolichyl monophosphate and its inhibition by tunicamycin (GlcNAc-1P transferase inhibitor) supported the concept that isoproterenol specifically stimulated protein N-glycosylation event(s) in the cell.
Texto completo:
DisponíveL
Índice:
IMSEAR (Sudeste Asiático)
Assunto principal:
Oligopeptídeos
/
Asparagina
/
Glicosilação
/
8-Bromo Monofosfato de Adenosina Cíclica
/
Bovinos
/
Endotélio Vascular
/
Alprostadil
/
Divisão Celular
/
Células Cultivadas
/
Processamento de Proteína Pós-Traducional
Idioma:
Inglês
Ano de publicação:
1993
Tipo de documento:
Artigo
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