Stability and kinetic behaviour of some enzymes in surfactant environment.
Indian J Biochem Biophys
;
1989 Dec; 26(6): 390-3
Artigo
em Inglês
| IMSEAR
| ID: sea-27534
ABSTRACT
The interaction of alpha-chymotrypsin, invertase, alcohol dehydrogenase and alkaline phosphatase with some ionic and non-ionic surfactants, viz. sodium dodecyl sulphate, dioctyl sodium sulphosuccinate, hexadecyltrimethylammonium bromide, tetradecyltrimethylammonium bromide and Triton X-100, has been examined by studying the effect of varying surfactant concentrations on enzyme activities as well as by determining the time-dependent inactivation and the time-independent inhibition. The kinetic parameters, Km and Vmax, for alpha-chymotrypsin-catalysed reaction in presence of sodium dodecyl sulphate were evaluated. Anionic surfactants markedly decreased enzyme activity, whereas cationic surfactants were less effective. Nonionics showed no effect. This change in enzyme activity was also dependent on the nature of enzyme.
Texto completo:
DisponíveL
Índice:
IMSEAR (Sudeste Asiático)
Assunto principal:
Tensoativos
/
Álcool Desidrogenase
/
Estabilidade Enzimática
/
Cinética
/
Quimotripsina
/
Beta-Frutofuranosidase
/
Fosfatase Alcalina
/
Glicosídeo Hidrolases
/
Animais
Idioma:
Inglês
Revista:
Indian J Biochem Biophys
Ano de publicação:
1989
Tipo de documento:
Artigo
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