Nucleic acid binding properties of a helix stabilising nucleoid protein from the thermoacidophilic archaeon Sulfolobus acidocaldarius that condenses DNA into compact structures.
Indian J Biochem Biophys
;
1995 Dec; 32(6): 447-55
Artigo
em Inglês
| IMSEAR
| ID: sea-27625
ABSTRACT
Helix stabilising nucleoid protein (HSNP-C') from an acidothermophilic archaeon Sulfolobus acidocaldarius has been characterised with respect to interaction with nucleic acids by gel retardation assay, binding to nucleic acid columns, fluorescence titrations and electron microscopy. The protein exists in solution as very large multimeric aggregates as indicated by cross-linking studies. The protein binds strongly and co-operatively to double stranded DNA. Electron microscopy of the complexes of the protein with DNA shows compact structures suggesting that the protein condenses DNA.
Texto completo:
DisponíveL
Índice:
IMSEAR (Sudeste Asiático)
Assunto principal:
Proteínas de Bactérias
/
DNA Bacteriano
/
Sulfolobus acidocaldarius
/
Proteínas de Ligação a DNA
/
Conformação de Ácido Nucleico
Idioma:
Inglês
Revista:
Indian J Biochem Biophys
Ano de publicação:
1995
Tipo de documento:
Artigo
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